Figure 5.

Efficacy of PEA-OXA on caspase 3 expression and LDH levels (induced by transient MCAo). An immunohistochemical analysis of caspase 3 (E) was performed. A marked increase in its expression was detected in brain samples from animals administered vehicle (B) compared to the sham-treated animals (A). This expression was notably reduced by treatment with PEA-OXA (D), while PEA treatment displayed less efficacy (C). PEA-OXA administration also reduced LDH levels to normal levels (F). For the immunohistochemistry, a 20× magnification is shown (50-µm scale bar). A p-value of less than 0.05 was considered significant: ° p < 0.05 versus vehicle; *** p < 0.001 versus sham; °°° p < 0.001 versus vehicle; # p < 0.05 versus PEA; ## p < 0.01 versus PEA.