Figure 1.

Thermostability of the various C. thermocellum BglA mutants. Wild-type (WT) C. thermocellum BglA and the different mutants were incubated at 66–72 °C for 1 h, followed by activity assay (using p-nitrophenyl-β-d-1,4-glucopyranoside [pNPG], an analogue of the natural β-glucosidase substrate). Residual activity of the different mutants (defined as the activity of the heat-shocked enzyme × 100/activity of the non-heated enzyme) was calculated. Mut 1 and A17S demonstrated similar thermostability.