Figure 5.

Hydrolysis of microcrystalline cellulose by the C. thermocellum secretome. Solutions containing the C. thermocellum secretome were applied on Avicel with or without the addition of BglA (wild-type or mutant), followed by incubation at either 60 or 70 °C for 48 h. The concentration of the released soluble glucose was measured by HPLC. Mut 1 showed higher glucose yields at both temperatures, with significant advantage at 70 °C. The experiment was conducted in triplicate. Bars indicate standard deviation.