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I and II, equatorial-oriented methyl groups of 4,6 galacto- and manno-pyranosyls. III, identification of the attachment site of pyruvylation via hetero multiple bond correlation (HMBC). IV, through-space correlation of the pyruvate methyl group to a ring proton—in this case H3. Pink arrows indicate though-bond interactions of neighbouring protons (HMBC), while blue arrows indicate through-space interactions of neighbouring protons (NOE).
