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. 2019 Sep 28;20(19):4826. doi: 10.3390/ijms20194826

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Exportin 1 (XPO1) is an essential protein in gastric cancer. (A) Left column: overall survival rate of gastric cancer patients with XPO1 expression levels at various stages of disease. Middle column: survival of gastric cancer patients (male) with XPO1 expression levels at various stages of disease. Right column: survival of gastric cancer patients (female) with XPO1 expression levels at various stages of disease. (B) A panel of gastric cancer tissues from different disease stages were stained for XPO1 expression levels using immunohistochemistry. Graph quantification of scoring is provided below. (C) Western blot of a panel of gastric cancer cell lines probed for XPO1 and beta-actin. (D) Western blot of XPO1 silenced NCI-N87 cells and beta-actin. (E) Growth analysis of silenced XPO1 in NCI-N87 cells (n = 3). ** p < 0.01. (F) Colony formation assay NCI-N87 cells (n = 3) post XPO1 siRNA.