Skip to main content
. 2019 Sep 22;20(19):4697. doi: 10.3390/ijms20194697

Figure 1.

Figure 1

RhoE expression is elevated during transforming growth factor-β (TGF-β)-mediated epithelial-to-mesenchymal transition (EMT) in HeLa cells. (A) Protein levels of RhoE, fibronectin, and ZO-1 in HeLa cells after treatment with 1 ng/mL TGF-β1. Whole-cell lysates were subjected to Western blot analysis and β-actin was used as a loading control. The three lanes represent triplicates using cell lysates prepared from different culture plates. Signal intensities of the proteins were quantified using NIH-Image software. Data in each column represent the mean and standard deviation of three independent experiments. (B) qPCR analysis of RhoE expression in HeLa cells treated with TGF-β1. Expression levels of RhoE were normalized to 18S rRNA expression. Data in each column represent the mean and standard deviation of three independent experiments. The statistical significance of differences between two groups was evaluated using a two-tailed Student’s t-test. * p < 0.01 and ** p < 0.05.