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. 2019 Sep 22;20(19):4697. doi: 10.3390/ijms20194697

Figure 3.

Figure 3

Knockdown of RhoE expression changes the expression levels of EMT-related genes in HeLa cells. (A) Protein expression of fibronectin, snail, and ZO-1 in HeLa cells transfected siRhoE-A. Cells were treated with 1 ng/mL TGF-β1 for 72 h. Whole-cell lysates were subjected to Western blot analysis and β-actin was used as a loading control. The three lanes represent triplicates using cell lysates prepared from different culture plates. Signal intensities of the proteins were quantified using NIH-Image software. Each column represents the mean and standard deviation of three independent experiments. (B) qPCR analysis of fibronectin and snail expression in RhoE knockdown cells. Expression levels of fibronectin and snail were normalized to 18S rRNA expression. Each column represents the mean and standard deviation of three independent experiments. Statistical significance was assessed using one-way ANOVA with a post hoc Tukey–Kramer HSD test. *p < 0.01 and **p < 0.05.