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. 2019 Sep 22;20(19):4697. doi: 10.3390/ijms20194697

Figure 4.

Figure 4

Knockdown of RhoE expression enhances the migration of HeLa cells undergoing TGF-β-mediated EMT. HeLa cells were transfected with siRhoE-A or a control siRNA and treated with 1 ng/mL TGF-β1 for 72 h. Cells undergoing EMT were plated in the upper chamber of the filters coated with fibronectin. Cells that migrated to the underside of the transwell insert were measured after 24 h. (A) Representative images of migrated cells are shown. (B) The mean number of migrated cells in the field was calculated. Data in each column represent the mean and standard deviation of three independent experiments. (C) Cells undergoing EMT were plated in culture plates. After 24 h, cells were trypsinized and counted. Data in each column represent the mean and standard deviation of three independent experiments. Statistical significance was assessed using one-way ANOVA with a post hoc Tukey–Kramer HSD test. *p < 0.01.