Table 1.
Multivariate analysis of variance (MANOVA) and univariate ANOVA for FA values (C16:0 Palmitic, C18:0 Stearic, C18:1 Oleic, C18:2 Linoleic, C20:0 Arachidic) represent proportion of total FA for the specific FAs.
| Trait | Source of variation | Wilk’s λ | df | Num df | den df | p |
|---|---|---|---|---|---|---|
| All | Genotype | 0.01 | 419 | 2514 | 5331 | <0.0001 |
| All | Pollination type | 0.51 | 2 | 12 | 1774 | <0.0001 |
| All | Harvest season | 0.64 | 1 | 6 | 887 | <0.0001 |
| SS | df | MS | F | Holm p | ||
| C16:0 | Genotype | 1856.13 | 419 | 4.43 | 4.36 | <0.0001 |
| C16:0 | Pollination | 547.69 | 2 | 273.85 | 1.29 | 0.00324 |
| C16:0 | Harvest season | 4.23 | 1 | 4.23 | 0.01 | 0.9999 |
| C16:0 | Residuals | 905.73 | 892 | 1.02 | ||
| C18:0 | Genotype | 1955.74 | 419 | 4.67 | 856.75 | <0.0001 |
| C18:0 | Pollination | 459.60 | 2 | 229.80 | 201.34 | <0.0001 |
| C18:0 | Harvest season | 179.26 | 1 | 179.26 | 78.53 | <0.0001 |
| C18:0 | Residuals | 1018.11 | 892 | 1.14 | ||
| C18:1 | Genotype | 779.32 | 419 | 1.86 | 964.08 | <0.0001 |
| C18:1 | Pollination | 52.12 | 2 | 26.06 | 64.48 | <0.0001 |
| C18:1 | Harvest season | 165.45 | 1 | 165.45 | 204.68 | <0.0001 |
| C18:1 | Residuals | 721.05 | 892 | 0.81 | ||
| C18:2 | Genotype | 274.54 | 419 | 0.66 | 3.51 | <0.0001 |
| C18:2 | Pollination | 3.02 | 2 | 1.51 | 0.04 | 0.9999 |
| C18:2 | Harvest season | 5.95 | 1 | 5.95 | 0.08 | 0.9999 |
| C18:2 | Residuals | 166.52 | 892 | 0.19 | ||
| C20:0 | Genotype | 5.23 | 419 | 0.01 | 591.82 | <0.0001 |
| C20:0 | Pollination | 0.12 | 2 | 0.06 | 13.44 | <0.0001 |
| C20:0 | Harvest season | 0.74 | 1 | 0.74 | 83.7 | <0.0001 |
| C20:0 | Residuals | 3.94 | 892 | 0.00 | ||
| % Fat | Genotype | 2917.24 | 419 | 6.96 | 769.31 | <0.0001 |
| % Fat | Pollination | 4.72 | 2 | 2.36 | 1.24 | 0.5298 |
| % Fat | Harvest season | 429.78 | 1 | 429.78 | 113.34 | <0.0001 |
| % Fat | Residuals | 3382.49 | 892 | 3.79 |
Percent (%) fat is the percent of total fat in the bean. Tests for effects of genotypic variation (genotype), pollination (open pollinated, self-pollinated or pollen donor “SIAL 70”), and harvest season (two seasons), Wilk’s λ (multivariate test statistic), degrees of freedom (df), numerator (num df), and denominator degrees of freedom (den df), type III sum of squares, mean square (MS), F statistic (F value), and Holm’s adjusted P value (Holm p) for univariate ANOVA.