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. 2019 Oct 18;9:14947. doi: 10.1038/s41598-019-51351-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Free flight and landing preference in single male flies from various genotypes toward food labelled by CS males. Histograms represent (a) the frequency of flies taking upwind flight, (b) the overall landing frequency, and (c) the landing preference between plain food (PF; empty bars) and food labelled by CS males (coloured bars). In addition to CS control males (blue coloured bars), we tested the response of individual males derived from CS antibiotic-treated eggs (Antibio. CS males; green coloured bars), from the Zimbabwe30 wild-type line (Z30; yellow coloured bars), and from D. simulans (D. simulans; purple coloured bars). For parameters and statistics, please refer to the Fig. 2 legend. (a) G²_(3df) = 11.0, p = 0.012; (b) G²_(3df) = 7.9, p = 0.049; (c) Khi²_(3df) = 5.5, p = 0.142. The sample size is indicated at the bottom of each histogram bar (a).