Table 1.
Cryo-EM structure determination and model statistics.
| Data collection | |
| Magnification | 130 kx |
| Pixel size (Å) | 1.058 |
| Defocus Range (µm) | −0.85 to −1.65 |
| Voltage (kV) | 300 |
| Exposure time (s per frame) | 0.4 |
| Number of frames | 50 |
| Total dose (e/Å) | 86 |
| Reconstitution | |
| Box size (pixels) | 256 |
| Inter-box distance (Å) | 50.227 |
| Asymmetrical units | 11 |
| Micrographs | 153 |
| Manually picked filaments | 957 |
| Initial extracted segments | 9300 |
| Segments after 2D classification | 9270 |
| Resolution after 3D auto-refine (Å) | 3.1 |
| Final overall resolution (Å) | 2.8 |
| Estimated map sharpening B-factor (Å2) | −42.33 |
| Helical rise (Å) | 4.83 |
| Helical twist (°) | 65.45 |
| Atomic model | |
| GenBank Accession number | WP_068455577 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 1.148 |
| Validation | |
| Map CC (local) | 0.734 |
| MolProbity clashscore | 2.31 |
| Overall score | 1.05 |
| Rotamer outliers (%) | 0.00 |
| Ramachandran plot | |
| Favored (%) | 97.81 |
| Allowed (%) | 2.19 |
| Outliers (%) | 0.00 |