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. 2019 Oct 18;10:4764. doi: 10.1038/s41467-019-12708-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — SREF microscopy. a Energy diagram for SREF process. b Microscope setup for SREF imaging. c SREF spectrum of 5-μM Rh800 in DMSO. SREF spectrum (solid line) is fitted with a sum of Voigt shape with an exponential decay background (dashed line). d Normalized SREF peak as Voigt shape (solid line) and Lorentzian shape after laser broadening deconvolution (dashed line)