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. 2019 Oct 18;10:4764. doi: 10.1038/s41467-019-12708-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Water density inside live HeLa cells measured by SRS microscopy. a SRS image at frequency of 2940 cm⁻¹ (CH₃ stretching attributed mainly to protein) of live HeLa cells. b Spectral mapping of O–H stretching band at the nucleus and cytoplasm regions marked in (a) (n stands for nucleus and c stands for cytoplasm). The difference curve (shown on the right in percentage) is calculated as (nucleus−cytoplasm)/nucleus. c SRS image on integrated O–H stretching band over 3261–3424 cm⁻¹ (the gray area in (b), which is the spectral region that differs the most). Scale bar: 5 μm. The color scales display relative SRS signal level