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. 2019 Oct 18;10:4763. doi: 10.1038/s41467-019-12738-w

Fig. 1.

Fig. 1

Membrane binding by GAS7 as the flat two-dimensional sheet. a Giant unilamellar vesicles (GUVs) were incubated with 0.5 µM GFP-GAS7 F-BAR domain fragment or GAS7b. The proteins were incubated with GUVs containing PC, PE, PS and rhodamine-PE at a molar ratio of 20:20:60:0.02, and GUVs containing PC, PE, PS and PIP3 at a molar ratio of 40:40:20:5 were observed at 37 °C. Scale bar: 10 μm. b The time course of GFP-GAS7b assembly on GUVs. Scale bar: 5 μm. c Negatively stained transmission electron micrographs of the GAS7 F-BAR domain fragment, GAS7b, GAS7cb and GAS7b D207R mutant on the monolayered membrane. Negatively stained transmission electron micrograph of GAS7b alone and the membrane alone are also shown. Protein samples (0.1 μM) were incubated in the presence or absence of lipid monolayers containing PC, PE and PS at a molar ratio of 20:20:60. Scale bar: 100 nm. d Negatively stained transmission electron micrograph of the GAS7 F-BAR domain fragment in the monolayered membrane as in c for e. Scale bar: 20 nm. e The Fourier transform of the micrograph in d. Arrows indicate signals in the reciprocal space corresponding to the periodic striations in d with the periodic spacing of ~5 nm. A cross-section of the diffraction in the Fourier image is also shown. Arrows indicate the signals showing the regular spacing of ~5 nm