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. 2019 Oct 18;9:15007. doi: 10.1038/s41598-019-50941-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lack of interaction between the isolated ACD and either 14-3-3σ∆C (A) or the 14-3-3-phosphopeptide chimera pCH1. (B) These two scenarios, shown schematically on top of the panels, were examined by size-exclusion chromatography with simultaneous detection by 230 nm absorbance and Trp-specific fluorescence (excitation 298 nm, emission 360 nm). Concentrations of 14-3-3 and ACD are indicated in µM. Dashed lines show the positions of the main peaks on different profiles for clarity.