Table 1.
Structural parameters of the 14-3-3-pB6 chimera determined in the SEC-SAXS experiment.
| 14-3-3-pB6 chimera | |
|---|---|
| Guinier analysis | |
| I(0) (cm−1) | 652.5 ± 1.0 |
| Rg (nm) | 3.51 ± 0.01 |
| sRg range | 0.34 < sRg < 1.30 |
| p(r) analysis | |
| I(0) (cm−1) | 654.9 ± 1.0 |
| Rg (nm) | 3.57 ± 0.02 |
| Dmax (nm) | 12.5 |
| s range (nm−1) | 0.0969–2.2797 |
| CorMap P-value reciprocal space fit (GNOM estimate) | 0.32 |
| Volume, shape and molecular weight (MW) analysis | |
| Porod volume, nm3 | 141.4 |
| MW calculated from amino acid sequence, kDa | 41.7•2 = 83.4 |
| MW from SEC-MALLS and RI concentration, kDa (MW ratio) | 82.8 (0.99) |
| MW from Porod volume, kDa (MW ratio) | 88.1 (1.06) |
| MW from SAXSMOW, kDa (MW ratio) | 76.9 (0.92) |
| MW from Vc, kDa, (MW ratio) | 84.2 (1.01) |
| Hydrodynamic analysis (SEC-MALLS) | |
| Hydrodynamic radius, Rh (nm) | 4.18 |
| Rg / Rh ratio (compared to sphere) | 0.84 (0.78) |
| CRYSOL (15 harmonics, 101 points, constant enabled) | |
| s range for model fitting | 0.093–5 |
| χ2, CorMap P-value | 1.16, 0.027* |
| CORAL-derived model Rg (nm) | 3.50 |
*CRYSOL fit to the SAXS data for the best CORAL-derived model with the non-fixed 14-3-3/ACD interface. Note: GNOM, DATPOROD, DATMOW, DATVC, CORAL, CORMAP and CRYSOL can be found as part of the ATSAS 2.8 software package61.