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. 2019 Aug 13;28(11):1390–1403. doi: 10.1177/0963689719860826

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 3. — Progenitor/stem cell or saline injection. (A) Suprarenal aorta is identified (arrow) and overlying tissues dissected away. (B, C) A silk suture is held between the infrarenal aorta and inferior vena cava. Using mosquito forceps, a vascular clip is applied for infrarenal aorta clamping. (D) The left kidney is moved gently to the right side and kept covered with wet gauze. (E) Progenitor/stem cells or saline are injected into suprarenal aorta using an insulin needle (31-gauge) connected to Tygon plastic tubing and an insulin syringe. (F) Suprarenal aorta is compressed gently using a cotton stick after progenitor/stem cells or saline injection. (G) Verification that no bleeding arises from the suprarenal aorta (arrow). (H) A square piece of Surgicel Nu-Knit absorbable hemostat is added on top of the segment of the suprarenal aorta where progenitor/stem cells or saline were injected. (I, J) Abdominal suturing is performed in layers. Safety suturing is also performed in the lower, middle and upper thirds to avoid dehiscence (arrows).