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. 2019 Oct 18;10:4768. doi: 10.1038/s41467-019-12824-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Transitional B-1a cells are readily detected in neonatal spleens from Igll1^−/− mice. a Representative flow-cytometry plots showing transitional B-1a (CD19⁺CD93^lo/intIgM⁺B220^−/loCD5⁺) and transitional B-2 (CD19⁺CD93^lo/intIgM⁺CD5^−/loB220⁺) from wild-type and Igll1^−/−. The left plot is gated on CD19⁺ cells from D10–11 neonatal spleen. b Graphs represent the proportion of cells that are either transitional B-1a or transitional B-2. c Graphs show the total cell numbers of transitional B-2 or transitional B-1a in millions. For both b and c, the middle line in the boxplots represents the median. The box shows first and third quartiles, and the whiskers represent the max and min values. Each dot represents cells from a different individual mouse. Source data are provided as Source Data File