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. 2019 Oct 18;10:4768. doi: 10.1038/s41467-019-12824-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Igll1^−/− B-1a cells have a similar V_H gene segment V_H usage to wild-type controls. Representative synthesis graphs generated from IMGT/StatClonotype that compare differences in V gene segment usage between wild-type (blue) and Igll1^−/− (red)-derived B-1a cells (CD19⁺B220^loCD5⁺). The red box highlights V_H12 (V_H12-3) gene segment (n = 2 independent experiments). The graph combines a bar graph for the normalized proportions of each gene segment and the differences in proportions with significance and confidence intervals (CI)