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. 2019 Oct 18;9:14989. doi: 10.1038/s41598-019-51577-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EPO over-expression reduced expression of renal proteins involved in Ca²⁺ and phosphate transport in Tg6 mice. The protein expression of the (A) sodium phosphate co-transporter NaPi-IIa, (B) the epithelial Ca²⁺ channel TRPV5, and (C) the cytoplasmic Ca²⁺ -binding protein Calbindin-D28k protein was tested by immunoblotting of kidney extracts from 6–8 weeks old female Tg6 mice and their littermate controls. All membranes were stripped and reprobed for β-actin. Graphs show the ratio of the protein of interest over β-actin. Data are means ± s.e.m.; n = 5 for each group of mice. Significance was determined by unpaired t test and indicated as *p < 0.05 and **p < 0.01.