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. 2019 Oct 18;9:14954. doi: 10.1038/s41598-019-51004-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCS2 binds to a cell fraction with CSC characteristics. (A) ALDH⁺ H358 (sorted fraction) and human adipose mesenchymal stem cells (MSC) cells incubated with PSC2 bound beads. ALDH⁺ H358 cells have a higher affinity for PCS2-coated magnetic-beads than MSCs. (B–E) RT-qPCR analysis of expression of ALDH1A3, CD44, SOX2 and CD133 CSC markers in PSC2 coated magnetic bead-bound and unbound cells. In general, PCS2-bound fractions have higher expression of ALDH1A3, SOX2 and CD133 CSC specific biomarker genes, when detectable, compared to unbound fractions. Cell lines tested were (B) H358, (C) H1693, (D) H460, and (E) H1975. H460 and H1975 additionally had significantly higher CD44 expression on PCS2-bound fractions. (F,G) Colony formation of H358 cells separated using PCS2-coated magnetic-beads. PCS2 bound cells formed significantly more colonies after 2 weeks of growth than unbound or untreated fractions, indicating PCS2 is pulling down cells with CSC-like characteristics. Error bars represent standard deviation between triplicates. *Represents p value < 0.05.