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. 2019 Sep 24;20(19):4742. doi: 10.3390/ijms20194742

Figure 1.

Figure 1

Altered HIF1α-dependent effects of normoxia and hypoxia under different culture conditions. Western blot analysis was performed 24 h after the application of different treatments for HIF1α and carbonic anhydrase (CAIX), while β-actin served as a control. (Lane 1) Medium control; (lane 2) siRNA against HIF1α; (lane 3) 1% fetal bovine serum; (lane 4) 1% fetal bovine serum and siRNA against HIF1α; (lane 5) 5 mM glutamine; (lane 6) 5 mM glutamine and siRNA against HIF1α; (lane 7) 1% fetal bovine serum and 5 mM glutamine; and, (lane 8) 1% fetal bovine serum, 5 mM glutamine and siRNA against HIF1α under normoxic or hypoxic (*) conditions in MDA-MB-231 cells. The experiments were performed in RPMI 1640 in the presence of 11.1 mM glucose, but without glutamine. Deep sequencing analysis was performed for each of the corresponding RNA samples (lanes 7, 8, 7*, and 8*) from four independent experiments (Please see Supplemental Figure S3 and the results from the densitometric evaluation of the Western blots of four independent experiments).