Figure 2.

Effects of glutamine application on HIF1α expression in different tumor cell lines. Saos-2, XF354 and MDA-MB-231 cells were left untreated or treated with 5 mM glutamine in RPMI 1640 medium without glutamine under normoxic conditions for 24 h followed by Western blot analysis of HIF1α and β-actin as a loading control. Data from three independent experiments (Please see Supplemental Figure S4 and the results from the densitometric evaluation of the Western blots).