Figure 3.

Altered HIF1α expression after the application of acetylsalicylic acid or ibuprofen in different cell lines under normoxia. Saos-2, XF354 and MDA-MB-231 cells were treated with 10 mM acetylsalicylic acid or 3 mM ibuprofen in RPMI 1640 medium with 5 mM glutamine under normoxic conditions for 24 h. Western blot analysis was performed with anti-HIF1α monoclonal antibody and anti-β-actin monoclonal antibody as a control. Acetylsalicylic acid or ibuprofen significantly reduced the glutamine-induced expression level of the normoxic HIF1α. Data from three independent experiments (Please see Supplemental Figure S5 and for the densitometric evaluation of the Western blots). For the results of experiments under hypoxic conditions, please see Supplemental Figure S6.