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. 2019 Oct 18;10:4755. doi: 10.1038/s41467-019-12721-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — RNA-seq of human adult and fetal atria. a MA plot of left atrial and right atrial whole tissue expression (RPK). A total of 15,239 genes with RPKs > 1 are detected in both samples. b Volcano plot analysis of left versus right adult atrial expression. c Heatmap of left and right atria (n = 3) shows that these tissues cluster together. d Volcano plot of adult left atrial tissue versus PCM1-sorted left atrial nuclei (i.e., CM nuclei). e Heatmap of whole tissue (n = 3) and PCM1-enriched left atria (n = 2). f Volcano plot analysis of adult (n = 3) versus fetal (n = 2) atrial expression. g GO terms of top 500 expressed genes in fetal atria with rank and p-value. h GO terms of top 500 expressed genes in adult atria with rank and p-value. p-values were calculated using DAVID (based on Fischer-exact test) and Benjamini-Hochberg corrected. RPK reads per kilo base, PCM1 pericentriolar material 1, GO Gene Ontology