Fig. 3.

Frequency of viral RNA detection and virus isolation from foetal and maternal tissues. For each species, dam and foetus, results from RT-qPCR detection (detection limit 1.3 log10 RNA copies/ml plasma) and virus isolation (detection limit 2.3 log10 RNA copies/ml 10% organ suspension) are shown post necropsy. The time of necropsy varies between groups (ChAdOx1 RVF 23–24 DPC and mock-vaccinated 8–11 DPC). Placentomes are a combination of maternal and foetal tissue. Samples that were positive by RT-qPCR are colour coded as below, whilst green is used for those that were RT-qPCR negative. Grey shading represents samples that were not available due to autolysis. Samples from which infectious virus could be isolated are marked with an asterisk. Only positive RT-qPCR samples were subjected to viral isolation. N/A (not applicable): does number 241, 244 and 246 were pseudo-pregnant and hence had no foetuses for analysis