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Targeted deep sequencing and CRISPResso analysis. (a) PCR and sequencing of the Tmc1 gene. (b) Global indel analysis by CRISPREsso. Reads were not segregated. (c) Strategy to segregate Tmc1Bth reads and Tmc1WT reads. The region for splitting partly overlaps with indels, thus some reads cannot be assigned as mutant or WT. (d) Allele specific indel analysis by CRISPResso.
