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Background sequencing error vs. indel formation with SaCas9-KKH (mean ± SD). Background sequencing error rate (GFP only) and comparison indel events in SaCas9-KKH + gRNA 4.1/gRNA 4.2 and gRNA 4.3 transfected Tmc1WT/WT fibroblasts are plotted. The difference is not significant (two-tailed t-test, p=0.3).
