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. Author manuscript; available in PMC: 2019 Oct 21.
Published in final edited form as: Stem Cell Res. 2019 Sep 10;40:101573. doi: 10.1016/j.scr.2019.101573

Fig. 1.

Fig. 1.

Mitochondrial membrane potential decrease with differentiation. (A) HSC, MPP, Lin− and Lin+ bone marrow fractions were sorted and stained as described in the Material and Method section. TMRM intensity levels in HSCs, MPPs, Lin− and Lin+ fraction measured by flow cytometry and normalized on bone marrow mononuclear cells (BM-MNCs)’ TMRM intensity. (B) Representative gating strategy for sorting of 30% TMRM lowest (Lo) and 30% TMRM highest (Hi) intensity MPP, and same gating applied to HSC fraction. (C) Representative images and (D) quantification of % of ki67+ cells (labeled by grey circles) on total cells (DAPI) in sorted HSC, MPP, MPP-Hi and MPP-Lo fractions. (E) Quantification of ki67+ cells as a percentage of total cells in sorted HSC-Hi and HSC-Lo fractions. Error bars represent mean with SD. At least three mice were analyzed per each experiment and the sample size for flow data for each group is shown by dots. ANOVA test (A, D) and t-test (E) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. stands for no significant change.