Fig. 1.

e37a-Cacna1b splice isoforms are expressed in mPFC, vHPC and AMY. a Schematic showing the approximate location of PCR primers to amplify e37a-containing cDNA (horizontal arrows), the approximate location of a unique BsrGI site within the e37a sequence (vertical arrow), and the expected size for BsrGI digested products. b Representative image of an agarose gel electrophoresis of undigested PCR products of RT samples from mPFC, vHPC and AMY (lanes 1, 3, and 5 respectively). Negative controls for each RNA sample without reverse transcriptase (lanes 2, 4, and 6 respectively). Positive control with a plasmid that contains cDNA for e37a-Cacna1b (lane 7). BsrGI digested PCR products derived from RT-PCRs from mPFC, vHPC, AMY, and e37a-Cacna1b plasmid (lanes 8, 9, 10, 11 respectively)