Table 1.

Primers and expected products

Mouse line	Primers	Expected products
CCK-Cre	F-WT1: GGGAGGCAGATAGGATCACA F-MT1: TGGTTTGTCCAAACTCATCAA R: GAGGGGTCGTATGTGTGGTT	Hom: 180 bp Het: 180 bp and 468 bp WT: 468 bp
Dlx5/6-Flpe	F-T1: CAGAATTGATCCTGGGGAGCTACG R-T1: CCAGGACCTTAGGTGGTGTTTTAC F-C: CAAATGTTGCTTGTCTGGTG R-C: GTCAGTCGAGTGCACAGT TT	Transgene: 406 bp PCR positive control: 200 bp *PCR conditions do not differentiate between heterozygous and homozygous mice
Ai14 and RCFL-tdT	F-WT2: AAGGGAGCTGCAGTGGAG TA R-WT2: CCGAAAATCTGTGGGAAG TC R-MT1: GGCATTAAAGCAGCGTAT CC F-MT2: CTGTTCCTGTACGGCATGG	Hom: 196 Het: 196 and 297 WT: 297
CaMKIIα-Cre	F-T1: GTT CTC CGT TTG CAC TCA GG R-T1: CAG GTT CTT GCG AAC CTC AT F-C: AGT GGC CTC TTC CAG AAA TG R-C: TGC GAC TGT GTC TGA TTT CC	Transgene: ~  500 bp PCR positive control: 521 bp

All primers are reported in 5′ to 3′ direction