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. 2019 Sep 20;20(19):4665. doi: 10.3390/ijms20194665

Figure 1.

Figure 1

Collection of samples from leaf explant till embryo development covering 14 key sampled stages of the Arabica SE process: leaves from greenhouse plants (L1), explants during dedifferentiation [0 h (L2), 1 week (D1), 2 weeks (D2), 5 weeks (D3)], compact primary callus obtained 3 months after induction (C1), embryogenic callus obtained 7 months after induction (C2), established cell clusters obtained after 4 months in proliferation medium (C3), early redifferentiation from cell clusters [1 week in DIF medium without reducing cell density (R1), 24 h in DIF medium after reducing cell density (R2), 72 h (R3), 10 d (R4)], globular embryos (E1) and torpedo-shaped embryos (E2). Non-embryogenic callus (NEC) was also sampled at the same time as the embryogenic callus (C2). Images were taken using an Olympus E-5 digital camera mounted on an Olympus SZX7 stereomicroscope. The stereomicroscope was not used for (L1). Two types of calli can be seen in (D3): an organized yellowish callus (1) and a whitish spongy callus (2).