Table 7.
Hormones | Embryogenic Callus | Non-Embryogenic Callus |
---|---|---|
ABA | 130 ± 14 a | 8 ± 2 b |
ABAGE | 1200 ± 84 a | 24 ± 11 b |
ACC | 3074 ± 96 a | 3016 ± 161 a |
IAA | 25 ± 6 a | 29 ± 3 a |
IBA | N.D. a | N.D. a |
iP | N.D. a | N.D. a |
Z | 2 ± 1 a | N.D. b |
ZR | 40 ± 5 a | 10 ± 3 b |
Values represent the mean ± SD of 3 independent replicates, expressed in ng/g DW. Non-parametric two-by-two Wilcoxon mean comparison test was used to calculate any significant differences in hormone levels between embryogenic and non-embryogenic calli. Data followed by different letters are significantly different (P < 0.05). Grey color corresponds to a decrease in hormone levels in non-embryogenic calli compared to embryogenic-calli. N.D. stands for not detected. ABA: cis-abscisic acid, ABAGE: ABA glucose ester, IAA: indole-3-acetic acid, IBA: indole 3-butyric acid, iP: isopentenyladenine, Z: zeatin (cis- and trans-), ZR: zeatin riboside (cis- and trans-).