Table 1.
Specificities and sources of primary and secondary antibodies.
| Target (alternative name) [RRID number] | Conjugate | Species, isotype and dilution | Cells labeled | Company |
|---|---|---|---|---|
| CD11c [AB_2562807] | Armenian hamster monoclonal IgG 1:500 | Dendritic cell | Stemcell Technologies (Tullamarine, Australia) | |
| B220 (CD45R) [AB_312987] | Rat monoclonal IgG 1:300 (Karrinyup, Australia) | B cell | Australian Biosearch | |
| CD31 [AB_312897] | Rat monoclonal IgG 1:300 | Blood vessel endothelial cell | Australian Biosearch | |
| CD4 [AB_312709] | Rat monoclonal IgG 1:300 | T helper cell | Australian Biosearch | |
| CD8a [AB_312763] | Rat monoclonal IgG 1:300 | Cytotoxic T cell | Australian Biosearch | |
| Mac1 (CD11b) [AB_312785] | Rat monoclonal IgG 1:300 | Macrophage | Australian Biosearch | |
| Neurofilament 200 (NF-H) [AB_477272] | Rabbit polyclonal 1:4000 | Neuronal marker | Sigma (Castle Hill, Australia) | |
| Rabbit IgG H&L | Alexa Fluor® 555 | Goat polyclonal 1:1000 | Abcam Australia (Melbourne, Australia) | |
| Armenian Hamster IgG H&L | Alexa Fluor® 647 | Goat polyclonal 1:1000 | Abcam Australia | |
| Rat IgG H&L | Alexa Fluor® 488 | Goat polyclonal 1:1000 | Abcam Australia | |
| Rabbit IgG from serum (1:1000 of 2 mg/mL work solution) | Sigma |