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. 2019 Oct 14;13:1054. doi: 10.3389/fnins.2019.01054

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Copyright © 2019 Pathak, Srivastava, Padma, Gulati and Rajeswari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2D-DIGE image of PBMC proteins obtained from FRDA patients and healthy controls. Comparative PBMC proteomics of FRDA patients (n = 6) and age- and gender-matched healthy controls (n = 6) was done on 13-cm-long, pH 4–7 IPG strips using Cy3 (50 μg), Cy5 (50 μg), and pooled internal standard (50 μg). A total of 150 μg of total PBMC protein was used for isoelectric focusing (IEF) followed by second-dimension electrophoresis on SE 600 Ruby gel apparatus using 12.5% SDS-PAGE gel (16 × 13 cm) in dark. Gels were scanned by Typhoon variable mode imager for each Cy dye. Blue circles show protein spots that were up-regulated, and red circled spots were down-regulated in FRDA patients compared with matched healthy controls.