TABLE 2.

Forward and reverse primer sequences with amplicon length and melting temperature for genes validated by quantitative real-time PCR.

Name of the gene	Forward (F) and reverse (R) primer sequences (5′-to-3′ direction)	Amplicon length (bp)	Melting temperature (°C)
TF	F—GCTTACCTGGCTCCCAATAA R—CCACTATCCTTCTTCACCACAG	110	62 62
CAS8	F—TGCCTACAGGTTCCACTTCTG R—ATGATCGACCCTCCGCCAG	94	59.65 61.50
PDHE1	F—GTATGGATGAGGAGCTGGAAAG R—GCCCTCGACTAACCTTGTATG	93	62 62
ACTC1	F—CAGGGAGTTATGGTGGGTATG R—CCATGCTCGATGGGATACTT	101	62 62