Table 1.

Primers used to generate site-directed mutants by site-directed mutagenesis

Desired mutation	Sequence 5′ → 3′	Melting temperature (Tm)
A195S
RECQ1_583_FW	ACTCCAGAGAAAATTTCAAAAAGCAAAATGT	62.7
RECQ1_583_RV	ACATTTTGCTTTTTGAAATTTTCTCTGGAGT	62.7
R215Q
RECQ1_644_FW	CAAGGAGATTTACTCAAATTGCTGTGGATGA	65.1
RECQ1_644_RV	TCATCCACAGCAATTTGAGTAAATCTCCTTG	65.1
R455C
RECQ1_1363_FW	ATAAGCAAATGTCGTTGTGTGTTGATGGCTC	67.4
RECQ1_1363_RV	GAGCCATCAACACACAACGACATTTGCTTAT	67.4
M458K
RECQ1_1373_FW	GTCGTCGTGTGTTGAAGGCTCAACATTTTGA	69.1
RECQ1_1373_RV	TCAAAATGTTGAGCCTTCAACACACGACGAC	69.1
T562I
RECQ1_1685_FW	AAGACTACAGTTTTATAGCTTATGCTACCAT	60.8
RECQ1_1685_RV	ATGGTAGCATAAGCTATAAAACTGTAGTCTT	60.8