Figure 6.

NFATc1 rescued the impaired osteoclast differentiation of Stat3^Ctsk BMMs. A, BMMs from 4-week-old WT and Stat3^Ctsk mice were infected with lentiviruses expressing GFP or NFATc1 and then cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). TRAP staining was performed to evaluate osteoclast numbers. B, relative TRAP activity of culture supernatants from WT and Stat3^Ctsk BMMs infected with lentiviruses expressing GFP or NFATc1 and cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Five wells in each group were tested. Error bars represent mean ± S.D.; *, p < 0.05. C–E, Stat3 and osteoclast-specific gene expression of WT and Stat3^Ctsk BMMs infected with Lenti-GFP or Lenti-NFATc1 and cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days). Three independent cell isolations were collected and were tested three times. Error bars represent mean ± S.D.; *, p < 0.05. F, Western blotting assay of WT and Stat3^Ctsk BMMs infected with Lenti-GFP or Lenti-NFATc1 and cultured with M-CSF and RANKL until mature multinuclear osteoclasts formed (5–7 days).