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. 2019 Aug 29;294(42):15418–15434. doi: 10.1074/jbc.RA119.008615

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Figure 5. — C7orf43 is a TRAPPII complex component but is not required for the core complex integrity. A, immunoblotting of size-exclusion chromatography fractions performed using lysate from either siControl or siC7orf43(#1+#2)–treated HEK293 cells. Blots were probed with antibodies against C7orf43/TRAPPC14, TRAPPC proteins, and actin. Comparative protein ratios of siC7orf43/siControl were determined by densitometry and are shown below blots for expressing fractions. Representative results from two independent experiments are shown. B, immunoprecipitation analysis of transiently expressed TRAPPC10-LAP after siControl and siC7orf43/TRAPPC14(#1+#2) treatments in HEK293 cells. Blots were probed with antibodies against GFP and TRAPPC proteins. Protein levels compared with siControl in WCL and immunoprecipitations (IP) were determined by densitometry analysis and are shown below blots normalized for differences in GFP expression between RNAi treatments. Representative results from two independent experiments are shown. C, PSM and %C from MS analysis of immunoprecipitated TRAPPC10-LAP from transient expression in HEK293 cells after treatment with siControl or siC7orf43(#1+#2) for 72 h from a single experiment.