Figure 7.

Distal appendage proteins FBF1 and CEP83 are required for GFP-Rabin8 centrosomal accumulation and bind to TRAPPC14. A, plot showing quantification of GFP-Rabin8 centrosomal accumulation as described in Fig. 3D in GFP-Rabin8–expressing RPE-1 cells treated with siRNA against DAP proteins. Two-tailed, unpaired Student's t test was used. Means ± S.E. (error bars) are shown from three independent experiments with n > 150 cells in total. **, p < 0.01; ***, p < 0.001; n.s., not significant. B, immunoblotting of immunoprecipitated HA-luciferase or HA-TRAPPC14 with LAP-FBF1. Representative results from three independent FBF1 experiments performed in HEK293 are shown. C, model for TRAPPII complex and TRAPPC14 mediating vesicular Rabin8 delivery to MC for primary cilium formation.