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. 2019 Oct 17;13:3607–3623. doi: 10.2147/DDDT.S219074

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© 2019 Zhang et al.

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EGCG protected cardiomyocytes against H/R-Stimulated H9c2 cells injury through adjusting miRNA-384-5p to inactivate autophagy.

Notes: H9c2 cells were transfected with miR-384 mimic, miR-384 inhibitor. (A, B) Representative Western blotting results and quantitative data on autophagy-related protein expression in H9c2 cells following H/R in the presence or absence of miR-384. (C, D) Tandem mRFP-GFP-LC3B probe analysis of the autophagosome-lysosome fusion. Yellow dots represent the unfused autophagosome, whereas red dots denote the fusion, wherein autolysosomes are formed. H9c2 cells were transfected with a mRFP-GFP-LC3B adenovirus for 6 h and then subjected to various treatments, after which the dots were observed under a confocal fluorescence microscope. Magnification: 630×. Scale bar: 5μm. Bar graph showing the number of LC3 puncta per cell. At least 10 cells per group were counted randomly in three independent experiments. Data among groups were compared using one-way analysis of variance. Values are expressed as mean±SD (n=6). ANOVA testing was performed; * P<0.05, ** P<0.01.

Abbreviations: ANOVA, analysis of variance; EGCG, epigallocatechin gallate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; H/R, hypoxia/reoxygenation; LC3, light chain 3; miR-384, microRNA-384-5p; SD, Sprague-Dawley.