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. 2019 Oct 17;13:3607–3623. doi: 10.2147/DDDT.S219074

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© 2019 Zhang et al.

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miR-384 prevented the upregulation of Beclin-1 in H/R-stimulated H9c2 cells.

Notes: H9c2 cells were transfected with miR-384 mimic, miR-384 inhibitor, Beclin-1 OE and Beclin-1 KD. (A) Dual-luciferase gene reports experiments used to verify Beclin-1 was target gene of miR-384 in H9c2 cells. (B–E) The mRNA and protein levels of Beclin-1 were detected by qRT-PCR and Western blotting. (F) The mRNA levels of miR-384 were detected by qRT-PCR. Data among groups were compared using one-way analysis of variance. Values are expressed as mean±SD (n=6). ANOVA testing was performed; * P<0.05, ** P<0.01.

Abbreviations: ANOVA, analysis of variance; EGCG, epigallocatechin gallate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; H/R, hypoxia/reoxygenation; KD, knock-down; LC3, light chain 3; miR-384, microRNA-384-5p; mRNA, Messenger RNA; NC, negative control; OE, overexpression; SD, Sprague-Dawley.