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. 2019 Oct 14;10:1229. doi: 10.3389/fphys.2019.01229

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Copyright © 2019 Kimono, Sarkar, Albadrani, Seth, Bose, Mondal, Li, Kar, Nagarkatti, Nagarkatti, Sullivan, Janulewicz, Lasley, Horner, Klimas and Chatterjee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of claudin 2, occludin and aquaporin 3 in mouse small intestine. (A,D,G) Protein expression of occludin, claudin 2 and aquaporin 3 in mouse small intestine was determined by immunofluorescence microscopy and visualized at total 200X; scale 50 μm in tissues obtained from mice treated with vehicle control (CONT, n = 6); mice treated with GW chemicals (GW, n = 9) mice co-exposed with GW chemicals and antibiotics (GW + AB, n = 9) and mice treated with GW chemicals, Sparstolonin B (SsnB) and Sodium butyrate (GW + SsnB + BT) (n = 6). (C,F,I) Higher magnification images for GW chemical treated group (GW) focusing on the apical or apical-lateral membranes total magnification 600X; scale 20 μm). (B,E,H) Quantitative morphometric analysis of immunoreactivity of occludin or aquaporin 3 is represented as (% ROI) (^∗P < 0.05).