Table 1.
Target region | Coordinates relative to bregma (mm)b |
Number of animals |
|||
---|---|---|---|---|---|
A/P | M/L | D/V | rAAV-Cre | Mock | |
ARC | |||||
Single injection/side | −1.46 | +0.25 and −0.25 | −5.5 | 4 | 3 |
Multiple injections/side | −2.54 | +0.25 and −0.25 | −5.5 | 5 | |
−2.3 | −5.5 | ||||
−2.06 | −5.5 | ||||
−1.82 | −5.75 | ||||
−1.46 | −5.75 | ||||
DMH | −1.7 | +0.4 and −0.4 | −4.9 | 3 | |
PVN | −0.82 | +0.25 and −0.25 | −4.6 | 4 | |
LH | −1.46 | +1.1 and −1.1 | −4.75 | 5 | |
BLA | −1.34 | +3 and −3 | −4.4 | 2 |
aPresented are hypothalamic and extrahypothalamic regions, coordinates for stereotaxic injection, and the number of animals per group.
bAll injections were done bilaterally.