Fig. 2. Genetic ablation of Ano1 reduces Ca²⁺- and carbachol-induced Cl⁻ currents in colonocytes isolated from Cdx2-Ano1^fl/fl mice.

a Traces of whole-cell Cl⁻ currents induced by Ca²⁺ in colonocytes isolated from Ano1^fl/fl (WT) and Cdx2-Ano1^fl/fl (CKO) mice. Ca²⁺ (1 μM) was added to the pipette. The pipette solution contained 140 mM CsCl. The bath solution contained 140 mM NMDG-Cl. The holding potential was −60 mV. Whole-cell patches were formed at the beginning of the black bar. Niflumic acid, a blocker of ANO1, was applied to block the ANO1 current. (Right panel) Summary of the peak current density evoked by Ca²⁺ in WT and CKO colonocytes. **p < 0.01. b Traces of whole-cell Cl^– currents induced by carbachol in colonocytes from WT and CKO mice. After forming whole-cell patches, 1 mM carbachol was applied to the bath. (Right) Summary of the current density evoked by carbachol in WT and CKO colonocytes. *p < 0.05