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. 2019 Aug 5;51(8):91. doi: 10.1038/s12276-019-0287-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–e Immunofluorescence images of the jejunum (a–c) and colon (d, e) stained with antibodies specific for ANO1, as well as phalloidin and β-catenin, markers for apical and lateral membranes, respectively. Arrowheads indicate the strong expression of ANO1 in the apical membrane of villi and surface epithelial cells in the jejunum (a) and colon (d). ANO1 showed relatively weak expression in the apical membrane of the crypt in the jejunum and colon (arrows) compared to the villi and surface region (arrowheads). b, c, e Magnified images of epithelial cells in the square regions of (a) and (d). ANO1 was largely colocalized with phalloidin at the apical membrane (b) but was not colocalized with β-catenin, which is the lateral membrane marker (c)