Fig. 3. Inhibition of RIPK1 attenuated AP-related injury in rats.

a Representative images (4×) of hematoxylin and eosin-stained pancreatic tissues harvested from rats subjected to sham operation, AP, AP + si-RIPK1 or AP + si-NC for 24 h after AP induction. b TUNEL analysis of apoptotic cells and d the apoptosis index in pancreatic tissues harvested from the rats described in Fig. 3a. c Representative TEM images of pancreatic tissues from the rats described in Fig. 3a; bar: 2 μm. e EMSA analysis and f quantitation of NF-κB DNA-binding activity in pancreatic tissues harvested from the rats described in Fig. 3a. g Histopathological scoring was performed to evaluate pancreatic injury in the rats described above. The data are the means ± S.Ds. (n = 3). *P < 0.05 versus sham, ^P < 0.05 versus AP