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Primer sequence for methylation-specific PCR
| Gene | Primer sequence (5′–3′) |
|---|---|
| FOXA1methylation | F: TTTTATGAATGGTTTGGGTTTTTAC |
| R: ACGACTTAACGTACGAATAACTACG | |
| FOXA1—nonmethylation | F: TTTATGAATGGTTTGGGTTTTTAT |
| R: CAACTTAACATACAAATAACTACACT |
PCR polymerase chain reaction, FOXA1 forkhead box A1, F forward, R reverse
