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. 2019 Jul 8;51(7):75. doi: 10.1038/s12276-019-0267-6

Fig. 3. IRF1 is critical for the TNF-induced expression of CXCR3-binding chemokines and TNFSF13B.

Fig. 3

a–c FLSs were transfected with nontargeting (siCTRL) or IRF1-targeting siRNA pools. a Transfected FLSs from seven donors with RA were treated with TNF (10 ng/ml) for 6 h. Gene expression was determined by qPCR. Expression in the treated cells is presented relative to that in the unstimulated cells. Values are shown as the mean ± SEM. **p < 0.01, Wilcoxon matched-pairs test. b RA-FLSs were stimulated with TNF for 24 h. Representative western blots of at least four experiments with different RA-FLS cell lines are shown. c. IRF1 expression knockdown FLSs (n = 4) were treated with TNF (10 ng/ml) for 24 h. Supernatants were analyzed for CXCL10 expression by ELISA. Values are shown as the mean ± SEM. d qPCR was used to analyze gene expression in human RA-FLSs treated with TNF in the absence or presence of cycloheximide (CHX, 20 μg/ml). Each experiment was performed in technical triplicates (error bars, SEM of triplicates). Expression in the treated cells is presented relative to that in the unstimulated cells