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. 2019 Jul 10;51(7):1–14. doi: 10.1038/s12276-019-0272-9

Fig. 3. The effect of C646 on apoptosis and proliferation of pAVICs after calcification.

Fig. 3

a, b Flow cytometry analysis of apoptosis revealed that C646 inhibited apoptosis induced by high-calcium/high-phosphate treatment. The right bar graph shows the results of statistical analysis of the apoptosis ratios. c, d The expression of cleaved caspase 3 and Bcl-2 was assessed by western blotting. The relative protein levels were normalized to GAPDH levels. The data are shown as the means ± standard errors of the means of triplicates and are representative of three independent experiments performed (#p < 0.05, ###p < 0.001 compared with control; *p < 0.05, **p < 0.01 compared with high Ca+P)